Genechip expression analysis technical manual

Affymetrix GeneChip® expression arrays (Human Genome U Plus Array) were hybridized for 16 hours in a 45°C incubator, rotated at 60 rpm. According to the GeneChip® Expression Analysis Technical Manual (Rev. 5, Affymetrix Inc., Santa Clara, CA), the arrays were then washed and stained using the Fluidics Station and finally scanned. DNA microarray hybridization was conducted as described in the Affymetrix GeneChip Expression Analysis Technical Manual. Microarray analysis was performed using probe intensity data derived from the Mouse Gene ST Array. For the genes of interest, confirmation of gene expression was done using quantitative real-time PCR. SECTION 1 GeneChip® Expression Analysis Overview Introduction and Objectives Welcome to the Affymetrix GeneChip ® Expression Analysis Technical Manual. This manual is a technical guide for using GeneChip expression analysis probe arrays. All protocols included in this manual have been used successfully by scientists at Affymetrix, or have.

Washing and staining of arrays were performed using the GeneChip Fluidics Station with the EukGE-WS2v5_ protocol. Chips were scanned using the Affymetrix GeneChip Scanner Analysis of gene expression data. Image acquisition, quantification and data analysis were performed using the Affymetrix GeneChip Operating Software (GCOS) v Expression Analysis Technical Manual For HT Array Plates Using the GeneChip® Array Station (pdf, MB) Expression Analysis Technical Manual, with Specific Protocols for Use with the Hybridization, Wash, and Stain Kit (pdf, MB) Expression Wash, Stain and Scan User Manual for Cartridge Arrays (pdf, MB). The GeneChip Expression Analysis Technical Manual is designed for use in a system consisting of a Fluidics Station, a Hybridization Oven , and a Scanner. References 1. Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular Cloning: A Laboratory Manual, v.1 Cold Spring Harbor Laboratory Press, Cold.

Complete Manual: Expression Analysis Technical Manual (with hyperlinks) (zip, MB) Expression Analysis Technical Manual (for print) (zip, KB) Sections 1 - 4: (Manual Sections) Section 1: GeneChip Expression Analysis Overview (pdf, KB) Section 2: Eukaryotic Sample and Array Processing (pdf, KB). GeneChip™ Expression Analysis Overview The following major steps outline GeneChip™ expression analysis: 1. Target Preparation 2. Target Hybridization 3. Fluidics Station Setup 4. Probe Array Washing and Staining 5. Probe Array Scan 6. Data Analysis. Manual. This manual is a technical guide for using GeneChip expression analysis probe arrays. All protocols included in this manual have been used successfully by scientists at Affymetrix, or have been recommended by our collaborators during the development of particular products. The field of mRNA gene expression monitoring is rapidly evolving and periodic technical updates to this manual will.