Gel extraction kit manual

QIAEX II Agarose Gel Extraction Protocol This protocol is designed for the extraction of bp to kb DNA fragments from –2% standard or low-melt agarose gels in TAE or TBE buffers. Notes: • The yellow color of Buffer QX1 indicates a pH ≤ • Add ethanol (96–%) to Buffer PE before use (see bottle label for volume). DNA ladder was run on a 2% agarose gel and 4 different fragment sizes ( bp, bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q following manufacturer’s recommended protocols. DNA was analyzed on a 2% TBE agarose gel with the respective companies eluate being. USER GUIDE PureLink® Quick Gel Extraction Kit For purifying DNA fragments from agarose gels Catalog Numbers K, K Revision Date 21 March Publication Part Number MAN

The GeneJET™ Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer. The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. Gel purification of DNA is a common technique for the isolation of specific fragments from reaction mixtures. However, most methods either fail to completely remove agarose or shear DNA which can lead to problems in downstream manipulations. The E.Z.N.A.® Gel Extraction Kit uses proprietary chemistry and HiBind® technology to recover DNA. QIAquick Gel Extraction Kits (50) () Catalog No. QIAquick Spin Columns 50 Buffer QG* 2 x 50 ml 2 x ml Buffer PE (concentrate) 2 x 10 ml 2 x 50 ml Buffer EB 15 ml 15 ml Collection Tubes (2 ml) 50 Handbook 1 1 * Buffers PB, PN, and QG contain chaotropic salts which are irritants. Take appropriate laboratory safety measures.

The Thermo Scientific GeneJET Gel Extraction and DNA Cleanup Micro Kit is qualified by isolating 3 kb DNA fragment from µL reaction mixture and extracting 3 kb DNA from 1% agarose gel according the protocols outlined in the manual. The quality of the purified and. PCR clean-up, gel extraction Reagents, consumables, and equipment to be supplied by user Reagents • 96– % ethanol Consumables • mL microcentrifuge tubes • Disposable pipette tips Equipment • Manual pipettors • Centrifuge for microcentrifuge tubes • Heating block, water bath, or thermomixer for gel extraction. The QIAEX II and QIAquick Gel Extraction Kit can be used to extract DNA from polyacrylamide gels. The QIAEX II Handbook contains a protocol for Polyacrylamide Gel Extraction. A specialized User-Developed Protocol (QQ05) is available when using the QIAquick Gel Extraction Kit for this purpose.